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The delineation of distinct structures and regions of the nervous system has been critical in investigating how it works.

For example, much of what neuroscientists have learned comes from observing how damage or "lesions" to specific brain areas affects behavior or other neural functions.

In vertebrates, the nervous system is segregated into the internal structure of the brain and spinal cord (together called the central nervous system, or CNS) and the routes of the nerves that connect to the rest of the body (known as the peripheral nervous system, or PNS).

The nematode Caenorhabditis elegans has been studied because of its importance in genetics.

In the early 1970s, Sydney Brenner chose it as a model system for studying the way that genes control development, including neuronal development.

Immunocytochemistry is a special case of histochemistry that uses selective antibodies against a variety of chemical epitopes of the nervous system to selectively stain particular cell types, axonal fascicles, neuropiles, glial processes or blood vessels, or specific intracytoplasmic or intranuclear proteins and other immunogenetic molecules, e.g., neurotransmitters.

Immunoreacted transcription factor proteins reveal genomic readout in terms of translated protein.

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